DAP, CT (DAP1, Death-associated protein 1, DAP-1)

Size

£655.00

SKU:: 352200
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Peptide corresponding to 19aa near the C-terminal of human DAP.
Format:: Supplied as a liquid in PBS, 0.02% sodium azide.
Extra Details:: The Death-associated protein (DAP) is a basic proline-rich 15kD protein that as a positive mediator of programmed cell death that is induced by interferon-gamma (1). DAP is also a direct substrate of mammalian target of rapamycin (mTOR), a serine/threonine kinase that regulates cell growth and cell cycle, and a negative regulator of autophagy. Under rich nutrient conditions, mTOR phosphorylates DAP at Ser3 and Ser51; under starvation conditions, these residues are dephosphorylated and DAP is converted into an active suppressor of autophagy (2). Applications: Suitable for use in Immunofluorescence, ELISA, Western Blot, Immunohistochemistry (paraffin). Other applications not tested. Recommended Dilution: Western Blot: 1-2ug/mI Optimal dilutions to be determined by the researcher. Positive Control: Human Small Intestine Lysate Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.