Cytochrome C (CYC, Cytochrome Ccytc)

Size

£659.00

SKU:: 352196
Purity:: Purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Chicken/Avian
Immunogen:: Purified bovine Cytochrome C
Format:: Supplied as a liquid in PBS, 0.02% sodium azide.
Extra Details:: Cytochrome c is an electron transport protein from mitochondria. It is released from mitochondria to cytoplasm during the early stages of apoptosis, prior to caspase activation, DNA fragmentation, and loss of membrane potential. The cytoplasmic cytochrome c is associated with Apaf-1 and caspase-9 to activate caspase-3 and other caspases. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:2000-1:10,000, do not use dry milk in blocking or dilution buffer in your assay, use BSA (3-5% in blocking and 1-3% in dilution buffers) Western Blot: 1:2000-1:10,000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.