351924
ST14 (Suppressor of Tumorigenicity 14 Protein, Matriptase)
Size
£581.00
- SKU:
- 351924
- Application:
- WB
- Purity:
- Purified by Protein A and peptide affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to surrounding Asn280 of human ST14/Matriptase.
- Format:
- Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 0.1mg/ml BSA, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
- Extra Details:
- Suppressor of tumorigenicity 14 protein (ST14), also known as matriptase, is a type II transmembrane protease (1,2). It is highly expressed in epithelia cells and important for the differentiation and homeostasis of epithelium (3). ST14 is synthesized as a single-chain precursor, and cleaved first at Gly149 in endoplasmic reticulum or Golgi. The resulting N-terminal and C-terminal fragments are non-covalently associated, and interact with hepatocyte growth factor activator inhibitor 1 (HAI-1), which facilitates the transportation of ST14 to the plasma membrane. Cell surface ST14 can be activated by autocleavage at Arg614 though an incompletely understood mechanism. HAI-1 inhibits activated ST14, and HAI-1 and ST14 complex can be shed from cell surface (4,5). In different context, ST14 has been reported either as a tumor suppressor or as a tumor promoter (6-8). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, incubate membrane with diluted primary antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice