351905

RUVBL1 (Pontin, RuvB-like 1, 49kD TATA Box-binding Protein-interacting Protein, 49kD TBP-interacting Protein, TIP49a, Pontin 52, Nuclear Matrix Protein 238, NMP 238, 54kD Erythrocyte Cytosolic Protein, TIP60-associated Protein 54-alpha, TAP54-alpha, INO80 Complex Subunit H, INO80H, NMP238, TIP49, TIP49A)

Size

£390.00

SKU:: 351905
Purity:: Purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to residues surrounding Glu428 of human Pontin/RUVBL1.
Clone:: D1L8J
Format:: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 0.1mg/ml BSA, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Extra Details:: Reptin/RuvBL2 and Pontin/RuvBL1 are closely related members of the AAA+ (ATPase associated with diverse cellular activities) superfamily of proteins, and are putatively homologous to bacterial RuvB proteins that drive branch migration of Holliday junctions (1). Reptin and Pontin function together as essential components of chromatin remodeling and modification complexes, such as INO80, TIP60, SRCAP, and Uri1, which play key roles in regulating gene transcription (1,2). In their capacity as essential transcriptional co-regulators, Reptin and Pontin have both been implicated in oncogenic transformations, including those driven by c-Myc, beta-catenin, and E1A (2-7). Applications: Suitable for use in Western Blot and ChIP. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, incubate membrane with diluted primary antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. ChIP: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice