351874
Nischarin, NT (Imidazoline Receptor Antisera- selected Protein, IRAS, NISCH)
Size
£626.00
- SKU:
- 351874
- Application:
- WB
- Purity:
- Purified
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to human Nischarin at N-terminal.
- Clone:
- D6T4X
- Format:
- Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 0.1mg/ml BSA, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
- Extra Details:
- Nischarin (also known as imidazoline receptor antisera-selected protein, IRAS) was first identified through its association with the cytoplasmic domain of integrin alpha5, and shown to regulate cell migration and cystoskeletal organization (1). Nischarin regulates Rac-1 signaling (2), as well as the p21-activated kinase (PAK) (3) and cofilin/LIMK pathways (4). Nischarin also interacts with LKB1, regulating the migration and metastatic behavior of breast epithelial cells (5). In addition, nischarin regulates neuronal migration in rat brain (6). Research studies have implicated nischarin in the regulation of invasion and metastasis of breast cancer (7,8). Researchers have shown that nischarin is frequently downregulated in ovarian cancer, and regulates invasion through focal adhesion kinase (FAK) signaling (9). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, incubate membrane with diluted primary antibody in 5% nonfat dry milk, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice