351867
MPC2 (Mitochondrial Pyruvate Carrier 2, Brain Protein 44, BRP44)
Size
£626.00
- SKU:
- 351867
- Purity:
- Purified
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to residues surrounding Asn33 of human MPC2.
- Clone:
- D4I7G
- Format:
- Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol
- Extra Details:
- The transport of the glycolytic end product pyruvate into mitochondria and the decarboxylation of pyruvate in the citric acid cycle generate energy through oxidative phosphorylation under aerobic conditions. Two inner mitochondrial membrane proteins, mitochondrial pyruvate carrier1 (MPC1) and mitochondrial pyruvate carrier 2 (MPC2) form a 150kD complex and are essential proteins in the facilitated transport of pyruvate into mitochondria. Mutations in the corresponding MPC1 gene are associated with deficient pyruvate transport, and may result in lactic acidosis, developmental delay, and premature death. Altered MPC1/MPC2 expres- sion or activity may result in significant metabolic disorders and contribute to the increase in aerobic glycolysis in cancer cells (a.k.a. the Warburg Effect). Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000, incubate membrane with diluted primary antibody in 5% nonfat dry milk, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice