351860
MEKK2 (Mitogen-activated Protein Kinase Kinase Kinase 2)
Size
£581.00
- SKU:
- 351860
- Purity:
- Purified by Protein A and peptide affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to residues surrounding Gly292 of human MEKK2.
- Format:
- Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 0.1mg/ml BSA, 150mM sodium chloride, 50% glycerol.
- Extra Details:
- Mitogen-activated protein kinase kinase kinase 2 (MEKK2/MAP3K2) belongs to the MAP3K family of Ser/Thr kinases. Research studies have demonstrated that MEKK2 plays a pivotal role in transducing mitogenic signals emanating from EGFR and FGF2R to JNK and ERK5 signaling cascades (1,2). Post-translationally MEKK2 is regulated through multiple mechanisms including: dimerization (3,4), ubiquitination (5,6), phosphorylation (7) and methylation (8). Research studies implicate dysregulation of MEKK2 signaling in breast carcinoma (9), colorectal carcinoma (10), and pancreatic ductal adenocarcinoma (8). Applications: Suitable for use in Western Blot, Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, incubate membrane with diluted primary antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice