EphA7 (Ephrin Type A Receptor 7)

Size

£626.00

SKU:: 351806
Purity:: Purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to residues surrounding Leu914 of human EphA7.
Format:: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 0.1mg/ml BSA, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Extra Details:: The Eph receptors are the largest known family of receptor tyrosine kinases (RTKs). They can be divided into two groups based on sequence similarity and on their preference for a subset of ligands. While EphA receptors bind to a glycosylphosphatidylinositol-anchored ephrin A ligand, EphB receptors bind to ephrin B proteins that have a transmembrane and cytoplasmic domain (1,2). Research studies have shown that Eph receptors and ligands may be involved in many diseases including cancer (3). Both ephrin A and B ligands have dual functions. As RTK ligands, ephrins stimulate the kinase activity of Eph receptors and activate signaling pathways in receptor-expressing cells. The ephrin extracellular domain is sufficient for this function as long as it is clustered (4). The second function of ephrins has been described as “reverse signaling”, whereby the cytoplasmic domain becomes tyrosine phosphorylated, allowing interactions with other proteins that may activate signaling pathways in the ligand-expressing cells (5). The EphA7 receptor preferentially binds ephrin-A5 as a ligand. This ligand-receptor interaction stimulates EphA7 signaling and induces apoptotic cell death through TNFR1 and caspase-8 pathway (6,7). EphA7 plays a critical role in organ development during neural tube closure, cortical dendritic development and spine maturation as well as urine tract insertion (8-10). Secreted EphA7 has been shown to promote somatic cell reprogramming through ERK activity reduction (11). Silencing of the secreted form of EphA7 is associated with germinal center B cell lymhomas. The secreted form of EphA7 has been proposed as a soluble tumor suppresor in lymphoma (12-14). Applications: Suitable for use in Western Blot, Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, incubate membrane with diluted primary antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunoprecipitation: 1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice