Cre Recombinase, NT (Cyclization Recombination) (HRP)

Size

£691.00

SKU:: 351791
Application:: WB
Purity:: Purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to bacteriophage-P1 Cre recombinase at N-terminal.
Clone:: D7L7L
Format:: Supplied as a liquid in 12mM sodium phosphate dibasic, pH 7.4, 136mM sodium chloride, 2.6mM potassium chloride, 2mg/ml BSA, 50% glycerol. Labeled with horseradish peroxidase (HRP)
Extra Details:: Cre recombinase is a bacteriophage-P1 enzyme required for maintenance of the phage genome as a monomeric plasmid in the lysogenic state (1,2). This enzyme mediates a site-specific recombination between two 34-base pair loxP sites. This reaction can be carried out in vitro, indicating that it does not require accessory factors (3). The Cre/Lox system has been used for a number of in vitro and in vivo applications including targeted gene deletions (4) and gene- specific humanized animal models (5). Resolution of the crystal structure of the Cre-Lox complex revealed that two Cre molecules interact with a single Lox site (6). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, incubate membrane with diluted primary antibody in 5% nonfat dry milk, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice