CENP-I (Centromere Protein I, CENP-I, FSH Primary Response Protein 1, Follicle-stimulating Hormone Primary Response Protein, Interphase Centromere Complex Protein 19)

Size

£626.00

SKU:: 351782
Application:: WB
Purity:: Purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to residues surrounding Asn41 of human CENP-I.
Clone:: D4D5K
Format:: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 0.1mg/ml BSA, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Extra Details:: Kinetochores are mitotic structures that form on centromeres and attach to mitotic spindle microtubules. Kinetochore attachment to microtubules regulates chromosome segregation and progression through mitosis. Unattached kinetochores signal to the spindle assembly checkpoint (SAC) machinery, arresting cells in mitosis (1). CENP-I is a centromere protein associated with the inner kinetochore, and required for progression through mitosis and the appropriate localization of other kinetochore-associated and checkpoint proteins, including CENP-C, the RZZ complex, MAD1 and MAD2 (2-4). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, incubate membrane with diluted primary antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice