Angiomotin (AMOT)

Size

£626.00

SKU:: 351761
Purity:: Purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to residues near Pro1049 of human Angiomotin.
Clone:: D2O4H
Format:: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 0.1mg/ml BSA, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Extra Details:: Angiomotin (AMOT) is a widely expressed cell junction protein initially identified through its ability to bind angiostatin. Alternative splicing results in two isoforms of angiomotin, the full- length p130 and the N-terminal truncated p80. These isoforms, along with angiomotin-like 1 (AmotL1) and angiomotin-like 2 (AmotL2) comprise the Motin protein family. Angiomotin is important in endothelial cell polarity, migration and blood vessel formation during development, as well as in signaling through small GTPases and the Hippo/YAP pathway (reviewed in 1). Research studies have shown that angiomotin expression regulates migration and proliferation of breast cancer cells (2,3). Applications: Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation. Other applications not tested. Recommended Dilution: Immunofluorescence (Immunocytochemistry): 1:1000 Western Blot: 1:1000, incubate membrane with diluted primary antibody in 5% nonfat dry milk, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunoprecipitation: 1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice