uSP15, Recombinant, Human, aa1-235, His-Tag (Ubiquitin C-terminal hydrolase 15 Isoform 3, UNPH-2, UNPH4)

Size

£512.00

SKU:: 351569
Molecular Weight:: 29.5
Purity:: ≥90% (SDS-PAGE)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 0.15M sodium chloride, 1mM DTT, 10% glycerol.
Extra Details:: USP15 is a member of the ubiquitin specific protease (USP) family of deubiquitinating enzymes. USP enzymes play critical roles in ubiquitin-dependent processes through polyubiquitin chain disassembly and hydrolysis of ubiquitin-substrate bonds. The protein associates with the COP9 signalosome, and also plays a role in transforming growth factor beta signalling through deubiquitination of receptor-activated SMAD transcription factors. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 2. Recombinant protein corresponding to aa1-235 from human USP15, fused to His-tag at N-terminal expressed in E. coli. Molecular Weight: ~29.5kD (258aa) Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAEGGAA DLDTQRSDIA TLLKTSLRKG DTWYLVDSRW FKQWKKYVGF DSWDKYQMGD QNVYPGPIDN SGLLKDGDAQ SLKEHLIDEL DYILLPTEGW NKLVSWYTLM EGQEPIARKV VEQGMFVKHC KVEVYLTELK LCENGNMNNV VTRRFSKADT IDTIEKEIRK IFSIPDEKET RLWNKYMSNT FEPLNKPDST IQDAGLYQGQ VLVIEQKNED GTWPRGPSTP KKPLEQSC Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice