UCHL3, Recombinant, Human, aa1-230, His-Tag (Ubiquitin carboxyl-terminal hydrolase isozyme L3, UCH-L3, Ubiquitin thioesterase L3)

Size

£512.00

SKU:: 351561
Molecular Weight:: 28.3
Purity:: ≥95% (SDS-PAGE)
Storage Conditions:: -20°C/-70°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 1mM DTT, 10% glycerol.
Extra Details:: Ubiquitin carboxyl-terminal hydrolase isozyme L3, also known as UCHL3, is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. UCHL3 play a role in the regulation of neuronal development and spermatogenesis and have been implicated in neurodegenerative diseases. UCHL3 protein is 54% identical to that of UCHL1. Source: Recombinant protein corresponding to aa1-230 from human uCHL3, fused to His-tag at N-terminal expressed in E. coli. Molecular Weight: ~28.3kD (250aa) Specific Activity: >3000pmole/min/ug, measured by the hydrolysis of Ubiquitin-AMC at pH 8.0, 37°C. Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MEGQRWLPLE ANPEVTNQFL KQLGLHPNWQ FVDVYGMDPE LLSMVPRPVC AVLLLFPITE KYEVFRTEEE EKIKSQGQDV TSSVYFMKQT ISNACGTIGL IHAIANNKDK MHFESGSTLK KFLEESVSMS PEERARYLEN YDAIRVTHET SAHEGQTEAP SIDEKVDLHF IALVHVDGHL YELDGRKPFP INHGETSDET LLEDAIEVCK KFMERDPDEL RFNAIALSAA Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice