NME4, Recombinant, Human, aa33-187, His-Tag (Nucleoside Diphosphate Kinase, Mitochondrial, NDK, NDPKD, nm23-H4, NM23D)

Size

£351.00

SKU:: 351396
Molecular Weight:: 19.6
Purity:: ≥90% (SDS-PAGE)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 0.2M sodium chloride, 40% glycerol.
Extra Details:: NME4, also known as nucleoside diphosphate kinase, mitochondrial, belongs to the NDK family. NME4 are ubiquitous enzymes that catalyze transfer of gamma-phosphates, via a phosphohistidine intermediate, between nucleoside and dioxynucleoside tri- and diphosphates. The enzymes are products of the nm23 gene family, which includes NME4. NME4 plays a major role in the synthesis of nucleoside triphosphates other than ATP. Source: Recombinant protein corresponding to aa33-187 from human NME4, fused to His-tag at N-terminal expressed in E. coli. Molecular Weight: ~19.6kD (176aa) Specific Activity: >120units/mg Unit Definition: The amount of enzyme that convert 1umole each of ATP and TDP to ADP and TTP/minute at pH 7.5 at 25°C in a couple system with PK/LDH. Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MPSWTRERTL VAVKPDGVQR RLVGDVIQRF ERRGFTLVGM KMLQAPESVL AEHYQDLRRK PFYPALIRYM SSGPVVAMVW EGYNVVRASR AMIGHTDSAE AAPGTIRGDF SVHISRNVIH ASDSVEGAQR EIQLWFQSSE LVSWADGGQH SSIHPA. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice