Macrophage Colony-stimulating Factor 1, Recombinant, Human, aa33-255, His-Tag (CSF1, M-CSF, MCSF, Lanimostim)

Size

£544.00

SKU:: 351357
Molecular Weight:: 26.1
Purity:: ≥95% (SDS-PAGE)
Storage Conditions:: -20°C/-70°C
Supplier:: United States Biological
Format:: Supplied as a liquid in PBS, pH 7.4, 10% glycerol.
Extra Details:: CSF1, also known as macrophage colony-stimulating factor 1, is a hematopoietic growth factor that is the primary regulator of the survival, proliferation, differentiation and function of mononuclear phagocytes. It is a four alpha-helical bundle cytokine and its active form is found extracellularly as a disulfide linked homodimer. This protein plays important roles in innate immunity, cancer and inflammatory diseases, including systemic lupus erythematosus, arthritis, atherosclerosis and obesity. In several conditions, activation of macrophages involves a CSF1 autocrine loop. In addition, secreted and cell-surface isoforms of CSF1 can have differential effects in inflammation and immunity. Recombinant protein corresponding to aa33-255 from human CSF1, fused to His-tag at C-terminal expressed in insect cell. Molecular Weight: ~26.1kD (231aa) Amino Acid Sequence: EEVSEYCSHM IGSGHLQSLQ RLIDSQMETS CQITFEFVDQ EQLKDPVCYL KKAFLLVQDI MEDTMRFRDN TPNAIAIVQL QELSLRLKSC FTKDYEEHDK ACVRTFYETP LQLLEKVKNV FNETKNLLDK DWNIFSKNCN NSFAECSSQD VVTKPDCNCL YPKAIPSSDP ASVSPHQPLA PSMAPVAGLT WEDSEGTEGS SLLPGEQPLH TVDPGSAKQR PPRLEHHHHH H Biological Activity: Measured in a cell proliferation assay using M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED50 range <3ng/ml. Endotoxin: <1EU/1ug (LAL) Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice