Flt3 ligand, Recombinant, Human, aa27-184, His-Tag (Fms-related tyrosine Kinase 3 ligand, FLT3LG, Flt3L, FL, SL cytokine)

Size

£626.00

SKU:: 351224
Molecular Weight:: 19
Purity:: ≥95% (SDS-PAGE). Endotoxin: ≤1EU/ug (LAL).
Storage Conditions:: -20°C/-70°C
Supplier:: United States Biological
Format:: Supplied as a liquid in PBS, pH 7.4, 10% glycerol.
Extra Details:: FLT3LG, also known as fms-related tyrosine kinase 3 ligand, is an alpha-cytokine that promotes the differentiation of multiple hematopoietic cell lineages. Also, this protein acts as a growth factor that increases the number of immune cells by activating the hematopoietic progenitors. FLT3LG is crucial for steady-state pDC and cDC development. It controls the development of DCs and is particularly important for plasmacytoid DCs and CD8-positive classical DCs and their CD103-positive tissue counterparts. Recombinant protein corresponding to aa27-184 from human FLT3LG, fused to His-tag at C-terminal, expressed in Baculovirus (insect cell). Swiss/Unprot: P49771 Molecular Weight: ~19kD (166aa) Biological Activity: Measured in a cell proliferation assay using OCI-AML5 acute myeloid leukemia cells. The ED50 range is ≤5ng/ml. Amino Acid Sequence: TQDCSFQHSP ISSDFAVKIR ELSDYLLQDY PVTVASNLQD EELCGGLWRL VLAQRWMERL KTVAGSKMQG LLERVNTEIH FVTKCAFQPP PSCLRFVQTN ISRLLQETSE QLVALKPWIT RQNFSRCLEL QCQPDSSTLP PPWSPRPLEA TAPTAPQPLE HHHHHH Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice