DHFR, Recombinant, Mouse, aa1-187, His-Tag (Dihydrofolate reductase)

Size

£512.00

SKU:: 351189
Molecular Weight:: 23.6
Purity:: ≥95% (SDS-PAGE)
Storage Conditions:: -20°C/-70°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 2mM DTT, 0.1M sodium chloride, 10% glycerol.
Extra Details:: Dihydrofolate reductase (DHFR) is a member of the reductase family of enzymes that is ubiquitously expressed in all organisms. DHFR catalyzes the NADPH-dependent reduction of dihydrofolate to tetrahydrofolate, and it is essential for the synthesis of thymidylate, purines and several amino acids. Expression of methotrexate (MTX)- resistant variants of DHFR in normal hematopoietic cells is a potential strategy to permit administration of larger doses of MTX by alleviating drug toxicity in normal cells and tissues that are drug sensitive. Source: Recombinant protein corresponding to aa1-187 from mouse DHFR, fused to His-tag at N-terminal expressed in E. coli. Molecular Weight: ~23.6kD (207aa) Specific Activity: >2000pmol/min/ug, and is defined as the amount of enzyme that converts 1pmole of dihydrofolic acid to tetrahydrofolic acid/minute at pH 6.5 at 25°C. Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MVRPLNCIVA VSQNMGIGKN GDLPWPPLRN EFKYFQRMTT TSSVEGKQNL VIMGRKTWFS IPEKNRPLKD RINIVLSREL KEPPRGAHFL AKSLDDALRL IEQPELASKV DMVWIVGGSS VYQEAMNQPG HLRLFVTRIM QEFESDTFFP EIDLGKYKLL PEYPGVLSEV QEEKGIKYKF EVYEKKD Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice