CD14 (Monocyte differentiation Antigen CD14, Myeloid Cell-specific Leucine-rich Glycoprotein)

Size

£438.00

SKU:: 351135
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to human CD14, aa20-349, expressed in E. coli.
Format:: Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 10% glycerol.
Extra Details:: The protein encoded by this gene is a component of the innate immune system. CD14 exists in two forms, one anchored to the membrane by a glycosylphosphatidylinositol tail (mCD14), the other a soluble form (sCD14). Soluble CD14 either appears after shedding of mCD14 (48kD) or is directly secreted from intracellular vesicles (56kD). The x-ray crystal structure of human CD14 reveals a monomeric, bent solenoid structure containing a hydrophobic N-terminal pocket. CD14 was the first described pattern recognition receptor. CD14 acts as a co-receptor (along with the Toll-like receptor TLR4 and MD-2) for the detection of bacterial lipopolysaccharide (LPS). CD14 can bind LPS only in the presence of lipopolysaccharide-binding protein (LBP). Although LPS is considered its main ligand, CD14 also recognizes other pathogen-associated molecular patterns such as lipoteichoic acid. Applications: Suitable for use in ELISA, Western Blot and Flow Cytometry. Other applications not tested. Recommended Dilution: Western Blot: 1:500 Flow Cytometry: 1:100 Optimal dilutions to be determined by the researcher. Hybridoma: F0 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice