CBR1 (Carbonyl Reductase 1, Carbonyl Reductase 1, CBR, hCBR1, SDR21C1, CBR1, Carbonyl Reductase 1 15 Hydroxyprostaglandin dehydrogenase [NADP+], Carbonyl Reductase [NADPH] 1.CBR 1, CRN, NADPH Dependent Carbonyl Reductase 1, Prostaglandin 9 Ketoreductase, Prostaglandin E(2) 9 Reductase)

Size

£438.00

SKU:: 351127
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to human CBR1, aa1-277 expressed in E. coli.
Format:: Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 10% glycerol.
Extra Details:: Carbonyl reductase 1 (CBR1) is a NADPH-dependent, monomeric, and cytosolic enzyme belonging to a family of short-chain dehydrogenases/reductases. This protein consists of 277aa residues and is widely distributed in human tissues such as liver, epidermis, stomach, small intestine, kidney, neuronal cells, and smooth muscle fiber. CBR1 metabolizes many toxic environmental quinones and pharmacological relevant substrates such as the anticancer drug, doxorubicin. The best substrates of CBR1 are quinones, including ubiquinone-1 and tocophrolquinone (vitamin E). Applications: Suitable for use in ELISA, Western Blot, Immunocytochemistry, Immunofluorescence and Flow Cytometry. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000 Immunofluorescence (IC): 1;100 Flow Cytometry: 2-5ug/ml to label 1x10e6 cells Optimal dilutions to be determined by the researcher. Hybridoma: F0 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice