BCAT1 (Branched Chain Amino-acid transaminase Cytosolic, Branched Chain Amino-acid transaminase, Cytosolic, BCATC, BCT1, ECA39, MECA39, PNAS121, PP18)

Size

£438.00

SKU:: 351100
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to human BCAT1, aa1-386, expressed in E. coli.
Format:: Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 10% glycerol.
Extra Details:: BCAT1, also known as branched chain amino-acid transaminase (cytosolic form), catalyzes the reversible transamination of branched-chain alpha-keto acids to branched-chain L-amino acids essential for cell growth. Two different clinical disorders have been attributed to a defect of branched-chain amino acid transamination: hypervalinemia and hyperleucine-isoleucinemia. BCAT1 is expressed in the brain and kidney. Overexpressed in MYC-induced tumors such as Burkitt's lymphoma. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunocytochemistry/Immunofluorescence: 1:200 Flow Cytometry: 1:200 Optimal dilutions to be determined by the researcher. Hybridoma: F0 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.