ACTA2 (Actin aortic smooth Muscle, Actin, aortic smooth Muscle, AAT6, ACTSA, MYMY5)

Size

£438.00

SKU:: 351053
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to human ACTA2, aa3-377, expressed in E. coli.
Format:: Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 10% glycerol
Extra Details:: Alpha-actin-2 also known as actin, aortic smooth muscle or alpha smooth muscle actin (alpha-SMA, SMactin, alpha-SM-actin, ASMA). Actin proteins are major components of the eukaryotic cytoskeleton. At least six vertebrate actin isoforms have been identified. The cytoplasmic beta- and gamma-actin proteins are referred to as “non-muscle” actin proteins as they are predominantly expressed in non-muscle cells where they control cell structure and motility. Actin has major functions. Firstly, F-actin polymers form microfilaments - polar intracellular 'tracks' for kinesin motor proteins, allowing the transport of vesicles, organelles and other cargo. Actin is a component of the cytoskeleton and links to alpha-actinin, E-cadherin and beta-catenin at adherens junctions. actin has a role in cell motility through polymerization and depolymerization of fibrils. Applications: Suitable for use in ELISA, Western Blot and Flow Cytometry. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunocytochemistry/Immunofluorescence: 1:200 Flow Cytometry: 1:200 Optimal dilutions to be determined by the researcher. Hybridoma: F0 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice