UBE2F, Recombinant, Human, aa1-185, His-Tag (NEDD8-conjugating enzyme uBE2F)

Size

£512.00

SKU:: 350366
Molecular Weight:: 23.7
Purity:: ≥95% (SDS-PAGE)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 200mM sodium chloride, 1mM DTT, 30% glycerol.
Extra Details:: uBE2F, also Known as ubiquitin-conjugating enzyme E2 F, belongs to the ubiquitin-conjugating enzyme family and uBE2F subfamily. ubiquitin-conjugating enzymes more rarely as ubiquitin-carrier enzymes, perform the second step in the ubiquitination reaction that targets a protein for degradation via the proteasome. uBE2F accepts the ubiquitin-like protein NEDD8 from the uBA3- NAE1 E1 complex and catalyzes its covalent attachment to other proteins. Source: Recombinant protein corresponding to aa1-185 of human uBE2F, fused to His-tag at N-terminal expressed in E. coli. Molecular Weight: ~23.7kD Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMLTLAS KLKRDDGLKG SRTAATASDS TRRVSVRDKL LVKEVAELEA NLPCTCKVHF PDPNKLHCFQ LTVTPDEGYY QGGKFQFETE VPDAYNMVPP KVKCLTKIWH PNITETGEIC LSLLREHSID GTGWAPTRTL KDVVWGLNSL FTDLLNFDDP LNIEAAEHHL RDKEDFRNKV DDYIKRYAR Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.