PMVK, Recombinant, Human, aa1-192, His-Tag (Phosphomevalonate kinase)

Size

£512.00

SKU:: 350176
Molecular Weight:: 24.1
Purity:: ≥95% (SDS-PAGE)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 20mM Tris-HCl, pH 7.5, 0.1M sodium chloride, 1mM DTT, 10% glycerol.
Extra Details:: Phosphomevalonate kinase, also known as PMVK, is a cytosolic enzyme. It was expressed highly in heart, liver, skeletal muscle, kidney and pancreas and slightly lower in brain, placenta, and lung. Induced by sterol, PMVK participates in isopentenyl diphosphate biosynthesis via the mevalonate pathway. PMVK catalyzes the conversion of mevalonate 5-phosphate into mevalonate 5-diphosphate in the fifth reaction of the cholesterol biosynthetic pathway. Recombinant protein corresponding to aa1-192 of human PMVK, fused to a His-tag expressed in E. coli. Accession: NP_006547 Molecular Weight: ~24.1kD Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MAPLGGAPRL VLLFSGKRKS GKDFVTEALQ SRLGADVCAV LRLSGPLKEQ YAQEHGLNFQ RLLDTSTYKE AFRKDMIRWG EEKRQADPGF FCRKIVEGIS QPIWLVSDTR RVSDIQWFRE AYGAVTQTVR VVALEQSRQQ RGWVFTPGVD DAESECGLDN FGDFDWVIEN HGVEQRLEEQ LENLIEFIRS RL Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.