350173
PKM2 (Pyruvate Kinase Muscle Isoform M2, M2-PK)
Size
£435.00
- SKU:
- 350173
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to aa1-531 from human PKM2 expressed in E. coli. (NP_002645).
- Clone:
- 16C79
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 10% glycerol.
- Extra Details:
- PKM2 (pyruvate kinase M2), its association with phospho-tyrosine (pTyr) motifs, enables cells to use predominantly aerobic glycolysis instead of oxidative phosphorylation. Also PKM2 suggest that this change benefits cancer cells by enabling them to redirect glycolytic metabolites away from oxidation and energy production and, instead, toward anabolic processes and biosynthesis. PKM2-mediated generation of the aerobic glycolytic phenotype, although wasteful in terms of glucose consumption and waste generation, could provide a variety of advantages for cancerous cells. In addition nuclear translocation of the tumor marker pyruvate kinase M2 induces programmed cell death. Applications: Suitable for use in ELISA, Western Blot. and Immunofluorescence/Immunocytochemistry Other applications not tested. Recommended Dilutions: Western Blot: 1:1000 Immunofluorescence (IC): 1:250 Optimal dilutions to be determined by the researcher. Hybridoma: FO myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice