NT5M, Recombinant, Human, aa32-228, His-Tag (5' (3')-deoxyribonucleotidase, mitochondrial)

Size

£479.00

SKU:: 350136
Molecular Weight:: 25.1
Purity:: ~95% (SDS-PAGE).
Storage Conditions:: 0°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 0.1M sodium chloride, 1mM DTTS, 20% glycerol.
Extra Details:: NT5M, also known as DNT2, belongs to the 5'(3')-deoxyribonucleotidase family. It localizes to the mitochondrial matrix. This enzyme dephosphorylates the 5'- and 2'(3')-phosphates of uracil and thymine deoxyribonucleotides. The gene is located on chromosome 17 p11.2 in the critical region deleted in the Smith-Magenis syndrome (SMS), a genetic disease of unknown etiology. Recombinant protein corresponding to aa32-228 of human NT5M, fused to His-tag at N-terminal expressed in E. coli. Molecular Weight: ~25.1kD AA Sequence: MGSSHHHHHH SSGLVPRGSH MGGRALRVLV DMDGVLADFE GGFLRKFRAR FPDQPFIALE DRRGFWVSEQ YGRLRPGLSE KAISIWESKN FFFELEPLPG AVEAVKEMAS LQNTDVFICT SPIKMFKYCP YEKYAWVEKY FGPDFLEQIV LTRDKTVVSA DLLIDDRPDI TGAEPTPSWE HVLFTACHNQ HLQLQPPRRR LHSWADDWKA ILDSKRPC Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.