MED20, Recombinant, Human, aa1-212, His-Tag (Mediator of RNA polymerase II transcription subunit 20)

Size

£512.00

SKU:: 350070
Molecular Weight:: 25
Purity:: ≥90% (SDS-PAGE)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 20mM Tris-HCl, pH 8.5, 0.2M sodium chloride, 1mM DTT, 50% glycerol.
Extra Details:: MED20 is a subunit of the Mediator complex, a multiprotein coactivator of RNA transcription that interacts with DNA-bound transcriptional activators, RNA polymerase II and general initiation factors. Source: Recombinant protein corresponding to aa1-212 of human MED20, fused to His-tag at N-terminal expressed in E. coli. Molecular Weight: ~25kD Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMGVTCVS QMPVAEGKSV QQTVELLTRK LEMLGAEKQG TFCVDCETYH TAASTLGSQG QTGKLMYVMH NSEYPLSCFA LFENGPCLIA DTNFDVLMVK LKGFFQSAKA SKIETRGTRY QYCDFLVKVG TVTMGPSARG ISVEVEYGPC VVASDCWSLL LEFLQSFLGS HTPGAPAVFG NRHDAVYGPA DTMVQYMELF NKIRKQQQVP VAGIR Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.