HBQ1, Recombinant, Human, aa1-142, His-Tag (Hemoglobin subunit theta-1)

Size

£512.00

SKU:: 349973
Molecular Weight:: 17.9
Purity:: ≥90% (SDS-PAGE)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 0.15M sodium chloride, 1mM DTT, 10% glycerol.
Extra Details:: Hemoglobin subunit theta-1, also known as HBQ1, belongs to the Hemoglobin family. Hemoglobin (Hgb) is a 66.7kD protein coupled to four iron-binding, methenelinked tetrapyrrole rings (heme). The globin portion of Hgb consists of two alpha chains and two beta chains arranged in pairs forming a tetramer. Each of the four globin chains covalently associates with a heme group. The bonds between alpha and beta chains are weaker than between similar globin chains, thereby forming a cleavage plane that is important for oxygen binding and release. High affinity for oxygen occurs upon relaxation of the alpha1-beta2 cleavage plane. Source: Recombinant protein corresponding to aa1-142 of human HBQ1, fused to His-tag at N-terminal expressed in E. coli. Molecular Weight: ~17.9kD Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMALSAED RALVRALWKK LGSNVGVYTT EALERTFLAF PATKTYFSHL DLSPGSSQVR AHGQKVADAL SLAVERLDDL PHALSALSHL HACQLRVDPA SFQLLGHCLL VTLARHYPGD FSPALQASLD KFLSHVISAL VSEYR Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice