FBP2 (Fructose-16-bisphosphatase isozyme 2, Fructose-1,6-bisphosphatase isozyme 2)

Size

£438.00

SKU:: 349911
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to aa1-339 from human FBP2 expressed in E. coli.
Clone:: 16C45
Format:: Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 10% glycerol.
Extra Details:: FBP2 (Fructose-1,6-bisphosphatase isozyme 2) is a 339aa protein. FBP2 belongs to the FBPase class 1 family. The hydrolysis of fructose-1,6-bisphosphate to fructose-6-phosphate is a key reaction of carbohydrate metabolism. The enzyme that catalyzes this reaction appears to be present in all forms of living organisms. FBPase is encoded by two genes, FBP1 and FBP2, which express the liver and muscle isoforms, respectively. FBPase is regulated by AMP inhibition in most species. Inhibition of FBPase by AMP affects the turnover of bound substrate and not its affinity for substrate. Applications: Suitable for use in ELISA, Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:500-1:5000 Optimal dilutions to be determined by the researcher. Hybridoma: FO myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.