LOXL4, Recombinant, Human, aa570-756, His-Tag (Lysyl oxidase homolog 4, Lysyl oxidase-like 4, Lysyl oxidase-related protein C, LOXC)

Size

£823.00

SKU:: 349630
Molecular Weight:: 23
Purity:: Purified (~90%)
Storage Conditions:: -70°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 9mM Na2HPO4/KH2 PO4, pH 7.4, 124mM sodium chloride, 2.4mM potassium chloride, 10% glycerol.
Extra Details:: Recombinant protein corresponding to aa570-756 of human LOXL4, fused to His-tag at C-terminal; expressed in E. coli. Molecular Weight: ~23kD Assay Conditions: Enzymatic activity was confirmed by an AmplexRed/Peroxidase coupling method using 1,5-diaminopentane as a substrate. Purified LOXL4 (0.25-1ng/ul) was incubated with 3mM 1,5 diaminopentane, 0.4mM AmplexRed and 0.2unit/ul peroxidase in 100ul of the assay buffer (1.2M urea, 50mM borate, pH 8.8) for 15 min at 30°C. Fluorescence intensity is measured every 7 min at 530ex/590em. Applications: Suitable for use in the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.