Tumor Necrosis Factor, active (TNF), Recombinant, Rhesus Macaque

Size

£411.00

SKU:: 348269
Molecular Weight:: 17.3
Purity:: ≥95% as determined by SDS-PAGE and HPLC.
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Lyophilized from 0.2um filtered PBS, pH 7.4, 5% Trehalose
Extra Details:: Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.; The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells. {ECO:0000250}. Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by a cytotoxicity assay using murine L929 cells is less than 0.05ng/ml, corresponding to a specific activity of ＞ 2.0x10e7 IU/mg in the presence of actinomycin D. Sequence: VRSSSRTPSD KPVAHVVANP QAEGQLQWLN RRANALLANG VELTDNQLVV PSEGLYLIYS QVLFKGQGCP SNHVLLTHTI SRIAVSYQTK VNLLSAIKSP CQRETPEGAE AKPWYEPIYL GGVFQLEKGD RLSAEINLPD YLDFAESGQV YFGIIAL Molecular Weight: 17.3kD PubMed ID: 7561102; 15269276; Gene Name: TNF,TNFA,TNFSF2 Swiss Prot: P48094 Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice