Tumor Necrosis Factor, active (TNF), Recombinant, Human

Size

£411.00

SKU:: 348266
Molecular Weight:: 16.9
Purity:: ≥98% as determined by SDS-PAGE and HPLC.
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Lyophilized from 0.2um filtered 20mM PBS, 10mM Nacl, pH 7.0
Extra Details:: Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Impairs regulatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. Upregulates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key 'Ser-418' residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective (PubMed:23396208). Key mediator of cell death in the anticancer action of BCG-stimulated neutrophils in combination with DIABLO/SMAC mimetic in the RT4v6 bladder cancer cell line (PubMed:22517918). {ECO:0000269|PubMed:16829952, ECO:0000269|PubMed:22517918, ECO:0000269|PubMed:23396208}. The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells. {ECO:0000269|PubMed:16829952}. Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by a cytotoxicity assay using murine L929 cells is <0.01ng/ml, corresponding to a specific activity of ＞1x10e8 IU/mg in the presence of actinomycin D. Sequence: MRKRKPVAHV VANPQAEGQL QWLNRRANAL LANGVELRDN QLVVPSEGLY LIYSQVLFKG QGCPSTHVLL THTISRIAVS YQTKVNLLSA IKSPCQRETP EGAEAKPWYE PIYLGGVFQL EKGDRLSAEI NRPDYLDFAE SGQVYFGIIA F Molecular Weight: 16.9kD PubMed ID: 3555974; 6392892; 3883195; 2995927; 3856324; 3932069; 8499947; 10202016; 14656967; 15489334; 8631363; 8597870; 10205166; 2009860; 1402651; 9034191; 10e369255; 16829952; 16829951; 22517918; 23396208; 2922050; 1964681; 2551905; 9488135; 9442056; 12746914; 12915457 Gene Name: TNF, TNFA, TNFSF2 Swiss Prot: P01375 Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice