Pro-neuregulin-1, membrane-bound isoform, active (NRG1), Recombinant, Human

Size

£411.00

SKU:: 348207
Molecular Weight:: 7.5
Purity:: ≥97% as determined by SDS-PAGE and HPLC.
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Lyophilized from 0.2um filtered 1 × PBS, pH 7.4, with 5% Trehalose
Extra Details:: Direct ligand for ERBB3 and ERBB4 tyrosine kinase receptors. Concomitantly recruits ERBB1 and ERBB2 coreceptors, resulting in ligand-stimulated tyrosine phosphorylation and activation of the ERBB receptors. The multiple isoforms perform diverse functions such as inducing growth and differentiation of epithelial, glial, neuronal, and skeletal muscle cells; inducing expression of acetylcholine receptor in synaptic vesicles during the formation of the neuromuscular junction; stimulating lobuloalveolar budding and milk production in the mammary gland and inducing differentiation of mammary tumor cells; stimulating Schwann cell proliferation; implication in the development of the myocardium such as trabeculation of the developing heart. Isoform 10 may play a role in motor and sensory neuron development. {ECO:0000269|PubMed:1348215, ECO:0000269|PubMed:7902537}. Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using serum free human MCF-7 cells is <0.5ng/ml, corresponding to a specific activity of ＞2x10e6IU/mg. Sequence: SHLVKCAEKE KTFCVNGGEC FMVKDLSNPS RYLCKCPNEF TGDRCQNYVM ASFYKHLGIE FMEAE Molecular Weight: 7.5kD PubMed ID: 1350381; 7509448; 8096067; 7782315; 14702039; 16421571; 9333014; 12145742; 17565985; 15489334; 7689552; 1348215; 7902537; 10523851; 10597312; 10867024; 8062828; 8639490 Gene Name: NRG1, GGF, HGL, HRGA, NDF, SMDF Swiss Prot: Q02297 Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice