Interleukin-33, active (IL33), Recombinant, Human

Size

£518.00

SKU:: 348126
Molecular Weight:: 17.9
Purity:: ≥97% as determined by SDS-PAGE and HPLC.
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Lyophilized from 0.2um filtered 20mM PBS, 150mM NaCl, 1mM EDTA, pH7.4
Extra Details:: Cytokine that binds to and signals through the IL1RL1/ST2 receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells (PubMed:16286016). Involved in the maturation of Th2 cells inducing the secretion of T-helper type 2-associated cytokines. Also involved in activation of mast cells, basophils, eosinophils and natural killer cells. Acts as a chemoattractant for Th2 cells, and may function as an "alarmin", that amplifies immune responses during tissue injury (PubMed:17853410, PubMed:18836528). {ECO:0000269|PubMed:16286016, ECO:0000269|PubMed:17853410, ECO:0000269|PubMed:18836528}.; In quiescent endothelia the uncleaved form is constitutively and abundantly expressed, and acts as a chromatin-associated nuclear factor with transcriptional repressor properties, it may sequester nuclear NF-kappaB/RELA, lowering expression of its targets (PubMed:21734074). This form is rapidely lost upon angiogenic or proinflammatory activation (PubMed:18787100). {ECO:0000269|PubMed:18787100, ECO:0000269|PubMed:21734074}. Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine D10S cells is less than 0.05ng/ml, corresponding to a specific activity of ＞ 2.0x10e7 IU/mg. Sequence: SITGISPIT EYLASLSTYN DQSITFALED ESYEIYVEDL KKDEKKDKVLLSYYESQHPS NE SGDGVDGK MLMVTLSPTK DFWLHANNKE HSVELHKCEKPLPDQAFFVL HNMHSNCVSF ECKTDPGVFI GVKDNHLALI KVDSSENLCT ENILFKLSET Molecular Weight: 17.9kD PubMed ID: 12819012; 10566975; 16286016; 21454686; 14702039; 15164053; 15489334; 22307629; 17853410; 17185418; 18787100; 18836528; 19596270; 19559631; 19465481; 19439663; 21734074; 22215666; 19836339; 23980170 Gene Name: IL33,C9orf26,IL1F11,NFHEV Swiss Prot: O95760 Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice