Interleukin-1 receptor antagonist, active (IL1RN), Recombinant, Human

Size

£512.00

SKU:: 348097
Molecular Weight:: 17.1
Purity:: ≥96% as determined by SDS-PAGE and HPLC.
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Lyophilized from 0.2um filtered PBS, pH 7.4
Extra Details:: Inhibits the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. Has no interleukin-1 like activity. Binds functional interleukin-1 receptor IL1R1 with greater affinity than decoy receptor IL1R2; however, the physiological relevance of the latter association is unsure. {ECO:0000269|PubMed:7775431}. Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by inhibiting IL-1α- dependent proliferation of murine D10S cells is less than 40ng/ml, corresponding to a specific activity of ＞ 2.5x10e4 IU/mg in the presence of 50 pg/ml rHuIL-1α. Sequence: RPSGRKSSKM QAFRIWDVNQ KTFYLRNNQL VAGYLQGPNV NLEEKIDVVP IEPHALFLGI HGGKMCLSCV KSGDETRLQL EAVNITDLSE NRKQDKRFAF IRSDSGPTTS FESAACPGWF LCTAMEADQP VSLTNMPDEG VMVTKFYFQE DE Molecular Weight: 17.1kD PubMed ID: 2139180; 2137201; 1828896; 1385987; 8992991; 1827201; 7629520; 14702039; 15815621; 15489334; 2137200; 2143761; 9514884; 7989776; 7775431; 11991722; 8786086; 16335952; 19494218; 1534997; 8045306; 8175703; 7867645; 9062194; 18987736 Gene Name: IL1RN, IL1F3, IL1RA Swiss Prot: P18510 Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice