Interleukin-1 beta, active (IL1B), Recombinant, Human

Size

£518.00

SKU:: 348093
Molecular Weight:: 17.3
Purity:: ≥97% as determined by SDS-PAGE and HPLC. Endotoxin:≤1EU/ug (LAL).
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Lyophilized powder from 0.2um filtered PBS, pH 7.4. Reconstitute with sterile ddH2O, 0.1% BSA to a concentration of 0.1-1mg/ml.
Extra Details:: Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. {ECO:0000269|PubMed:3920526}. Full-lenth recombinant protein corresponding to aa117-269 from human mature IL1B, expressed in E. coli. Swiss/UniProt Accession: P01584. Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine D10S cells is less than 1.0pg/ml, corresponding to a specific activity of ＞1.0x10e9 IU/mg. Amino Acid Sequence: APVRSLNCTL RDSQQKSLVM SGPYELKALH LQGQDMEQQV VFSMSFVQGE ESNDKIP VAL GLKEKNLYLS CVLKDDKPTL QLESVDPKNY PKKKMEKRFV FNKIEINNKL EFESAQ FPNW YISTSQAENM PVFLGGTKGG QDITDFTMQF VSS Molecular Weight: 17.3kD PubMed ID: 6083565; 2989698; 3490654; 3493774; 2954882; 2635664; 11991722; 15815621; 15489334; 1919436; 3920526; 3281727; 1837236; 17431422; 3259176; 2585509; 2602367; 2372550; 2001363; 9062193; 20802483 Gene Name: IL1B,IL1F2 Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O, 0.1% BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice