Insulin-like Growth Factor I, Active (IGF1), Recombinant, Human, aa52-118

Size

£466.00

SKU:: 348072
Molecular Weight:: 7.4
Purity:: ≥97% (SDS-PAGE and HPLC.)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied a a lyophilized powder from PBS, pH 7.4.
Extra Details:: The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation. Partial recombinant protein corresponding to aa52-118 from human IGF1, expressed in E. coli. Swiss/Uniprot: P05019 Molecular Weight: ~7.4kD Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using serum free human MCF-7 cells is <2ng/ml. Specific Activity: >5x10e5 IU/mg. Amino Acid Sequence: TLCGAELVDA LQFVCGDRGF YFNKPTGYGS SSRRAPQTGI VDECCFRSCD LRRLEMYCAP LKPAKSA Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice