CD40 Ligand, active (CD40LG), Recombinant, Human

Size

£384.00

SKU:: 348018
Molecular Weight:: 16.3
Purity:: ≥95% (SDS-PAGE, HPLC). Endotoxin: ≤1.0EU per 1ug (LAL).
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a lyophilized powder from PBS, pH 7.0. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.
Extra Details:: Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching. Partial recombinant protein corresponding to aa113-261 from human CD40 Ligand, Tag-free expressed in E. coli. Swiss/UniProt Accession: P29965. Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of IL-8 production by human PBMC is less than 5-10ng/ml. Amino Acid Sequence: MQKGDQNPQI AAHVISEASS KTTSVLQWAE KGYYTMSNNL VTLENGKQLT VKRQGLYYIY AQVTFCSNRE ASSQAPFIAS LWLKSPGRFE RILLRAANTH SSAKPCGQQS IHLGGVFELQ PGASVFVNVT DPSQVSHGTG FTSFGLLKL Molecular Weight: 16.3kD PubMed ID: 1280226; 1385114; 7678782; 1281209; 7678552; 7999797; 15489334; 8626375; 11676606; 15193700; 8589998; 9605317; 7679206; 8094231; 7679801; 7717401; 7532185; 8889581; 8550833; 9150729; 9746782 Gene Name: CD40LG, CD40L, TNFSF5, TRAP Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice