Beta-defensin 103, active (DEFB103A), Recombinant, Human

Size

£411.00

SKU:: 347934
Molecular Weight:: 5.2
Purity:: ≥98% as determined by SDS-PAGE and HPLC.
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Lyophilized from 0.2um filtered 20mM PBS, pH 7.4, 130mM NaCl
Extra Details:: Exhibits antimicrobial activity against Gram-positive bacteria S.aureus and S.pyogenes, Gram-negative bacteria P.aeruginosa and E.coli and the yeast C.albicans. Kills multiresistant S.aureus and vancomycin-resistant E.faecium. No significant hemolytic activity was observed. {ECO:0000269|PubMed:11085990}. Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by anti-microbial activity against E.coli. is less than 30ug/ml, corresponding to a specific activity of ＞33.3 IU/mg. Sequence: GIINTLQKYY CRVRGGRCAV LSCLPKEEQI GKCSTRGRKC CRRKK Molecular Weight: 5.2kD PubMed ID: 11085990; 11702237; 11223260; 11741980 Gene Name: DEFB10e3A, BD3, DEFB10e3, DEFB3, DEFB10e3B Swiss Prot: P81534 Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.