Amphiregulin, active (AREG), Recombinant, Human, aa101-198

Size

£411.00

SKU:: 347929
Molecular Weight:: 11.3
Purity:: ≥95% as determined by SDS-PAGE and HPLC.. Endotoxin: ≤1EU/ug (LAL)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a lyophilized powder from PBS, pH 7.4. Reconstitute with sterile ddH2O to 0.1-1mg/ml.
Extra Details:: Ligand of the EGF receptor/EGFR. Autocrine growth factor as well as a mitogen for a broad range of target cells including astrocytes, Schwann cells and fibroblasts. Partial recombinant protein corresponding to aa101-198 from human AREG, expressed in E. coli. Amino Acid Sequence: SVRVEQVVKP PQNKTESENT SDKPKRKKKG GKNGKNRRNR KKKNPCNAEF QNFCIHGECK YIEHLEAVTC KCQQEYFGER CGEKSMKTHS MIDSSLSK PubMed ID: 2325643; 15489334; 2466334; 3413110; 2017164; 1333777; 22967896; 7679104; 17607000 Gene Name: AREG, SDGF, AREGB Swiss Prot: P15514 Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is between 5-10ng/ml. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.