PDGFB (Platelet-derived Growth Factor Subunit B, PDGF Subunit B, Platelet-derived Growth Factor B Chain, Platelet-derived Growth Factor beta Polypeptide, PDGF-2, Proto-oncogene c-Sis, Becaplermin, PDGF2, SIS)

Size

£510.00

SKU:: 327735
Purity:: Purified by affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to human PDGFB.
Format:: Supplied as a liquid in PBS, pH 7.3, 0.02% sodium azide, 50% glycerol.
Extra Details:: PDGF-BB is a cystine-linked homodimer PDGF family member with key roles in development, cell proliferation, cell survival, and angiogenesis. PDGF-BB is expressed by vascular endothelium, megakaryocytes and Leydig cells. PDGF-BB targets pericytes, fibroblasts, monocytes and other cell types. PDGF induces fibroblast growth and migration and is a chemoattractant for monocytes and granulocytes. Precursor PDGF-BB is cleaved intracellularly to generate a form that contains a carboxy-terminal stretch that serves to retain PDGF-BB in the extracellular matrix. In a second cleavage event, the carboxy-terminal stretch is removed extracellularly to generate mature PDGF-BB. PDGF-BB binds to PDGFRbeta and induces receptor dimerization. Signaling is through the PI3K/Akt, JNK, and PLCgamma pathways. PDGF-BB may have a role in some cancer types. Applications: Suitable for use in Western Blot, Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:500-1:2000 Immunohistochemistry: 1:50-1:200 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice