327627
Paxillin(Ab-31)
Size
£544.00
- SKU:
- 327627
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Peptide corresponding to the sequence around aa29-33 (T-P-Y-S-Y) derived from human Paxillin.
- Format:
- Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 0.02% sodium azide, 50% glycerol.
- Extra Details:
- An antiphosphotyrosine antibody was used to identify proteins that are phosphorylated in Rous sarcoma virus-transformed chick embryo fibroblasts, and a 76-kD protein was obtained that localizes to focal adhesions at the ends of actin-containing stress fibers in nontransformed cells (Ref.1). This protein was purified from chicken gizzard smooth muscle, and was named Pxn (Paxillin) ('paxillus' means 'small stake' or 'peg' in Latin) as a protein tethered to the membrane at focal adhesions Davidson D, et al. (2001) EMBO J; 20(13): 3414-3426 Kook S, et al. (2000) Mol Biol Cell; 11(3): 929-939 Fleming I, et al. (1999) Proc Natl Acad Sci U S A; 96(3): 1123-1128 Goldberg MB, et al. (2001) Microbiol Mol Biol Rev; 65(4): 595-626 Thomas JT, et al. (1999) Proc Natl Acad Sci U S A; 96(15): 8449-8454 Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:500-1:1000 Immunohistochemistry: 1:50-1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice