p27Kip1(Ab-10

Size

£544.00

SKU:: 327139
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Peptide corresponding to the sequence around aa8-12 (N-G-S-P-S) derived from human p27Kip1.
Format:: Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 0.02% sodium azide, 50% glycerol.
Extra Details:: Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Positive regulator of cyclin D-dependent kinases such as CDK4. Regulated by phosphorylation and degradation events. Le XF, et al. (2003)J Biol Chem. Connor MK, et al. (2003)Mol Biol Cell; 14(1): 201-13. McAllister SS, et al. (2003) Mol Cell Biol; 23(1): 216-28. Chopra S, et al. (2002)J Biol Chem; 277(36): 32413-6. Boehm M, et al. (2002)EMBO J; 21(13): 3390-401. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:500-1:1000 Immunohistochemistry: 1:50-1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.