322641
LTA
Size
£510.00
- SKU:
- 322641
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to human LTA
- Format:
- Supplied as a liquid in PBS, pH 7.3, 0.02% sodium azide, 50% glycerol.
- Extra Details:
- Lymphotoxin-alpha (LT-alpha), also known as TNF–beta, is a member of the TNF superfamily of proteins. NK cells, T cells, B cells, and lymphoid tissue-inducer cells express LT-alpha. LT-alpha can be secreted as a soluble homotrimer or form membrane bound heterotrimers with lymphotoxin-beta (LT alpha1beta2 or LT alpha2beta1) which can be cleaved from the cell surface by matrix metalloproteases. Soluble LT-alpha binds to and signals through TNFR1/TNFR2, activating the canonical NF-kappaB pathway. In contrast, LT alpha1beta2 heterodimers bind to the LTbetaR receptor and activate the noncanonical NF-kappaB pathway. As a result, LT-alpha and TNF-alpha have overlapping functions. Soluble LT-alpha and LT alpha1beta2 play key roles in lymphangiogenesis. The LT alpha1beta2/LTbetaR axis is essential for the development of lymphoid tissue. Applications: Suitable for use in Western Blot, Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:500-1:2000 Immunohistochemistry: 1:50-1:200 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice