320214
IL10
Size
£510.00
- SKU:
- 320214
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to human IL10
- Format:
- Supplied as a liquid in PBS, pH 7.3, 0.02% sodium azide, 50% glycerol.
- Extra Details:
- IL-10 is an anti-inflammatory cytokine that is produced by T cells, NK cells and macrophages. IL-10 initiates signal transduction by binding to a cell surface receptor complex consisting of IL-10 RI and IL-10 RII. Binding of IL-10 leads to the activation of Jak1 and Tyk2, which phosphorylates Stat-3. The anti-inflammatory activity of IL-10 is due to its ability to block signaling through other cytokine receptors, notably IFNgamma receptor, by upregulating expression of SOCS-1. In addition, IL-10 promotes T cell tolerance by inhibiting tyrosine phosphorylation of CD28. IL-10 is an important negative regulator of the immune response, which allows for maintenance of pregnancy. In contrast, increased IL-10 levels contribute to persistent Leishmania major infections. Applications: Suitable for use in Western Blot, Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:500-1:2000 Immunohistochemistry: 1:50-1:200 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice