HLA-DQB1/B2 (HLA Class II Histocompatibility Antigen, DQ beta 1 Chain, MHC Class II Antigen DQB1, HLA-DQB1, HLA-DQB, HLA Class II Histocompatibility Antigen, DQ beta 2 Chain, HLA Class II Histocompatibility Antigen, DX beta Chain, MHC Class II Antigen DQB2, HLA-DQB2)

Size

£364.00

SKU:: 319266
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to the Internal region of human HLA-DQB1/2.
Format:: Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Extra Details:: Destination of major histocompatibility complex (MHC) class II molecules for presentation to CD4+ helper T-cells is determined by two key events: the dissociation of class II-associated invariant chain peptides (CLIP) from an antigen binding groove in mhc ii-a/b dimers through the activity of MHC molecules HLA-DM and -DO, and subsequent peptide antigen binding. Accumulating in endosomal/lysosomal compartments and on the surface of B cells, HLA-DM, -DO molecules regulate the dissociation of CLIP and the subsequent binding of exogenous peptides to HLA class II molecules (HLA-DR, DQ, DP and DR) by sustaining a conformation that favors peptide exchange. RFLP analysis of HLA-DM genes from rheumatoid arthritis (RA) patients suggests that certain polymorphisms are genetic factors for RA susceptibility. The alpha 1 chain of HLA-DQ1 class II molecule complex can bind peptides and present them to CD4+ T lymphocytes. HLA-DQB1 may be implicated in multiple sclerosis. Applications: Suitable for use in Western Blot and ELISA. Other applications not tested. Recommended Dilution: Western Blot: 1:500-1:2000 ELISA: 1:20,000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice