EEF1B2 (Elongation Factor 1-beta, EF-1-beta, EEF1B, EF1B)

Size

£364.00

SKU:: 314405
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to the Internal region of human EEF1B2.
Format:: Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Extra Details:: EF-1 (elongation factor-1) is a multi-protein complex that is responsible for the delivery of aminoacyl-tRNAs to the ribosome. The EF-1 protein complex is composed of four subunits: EF-1 alpha, EF-1 beta, EF-1 delta and EF-1 gamma. EF-1 beta is a 225aa member of the EF-1 beta/EF-1 beta protein family and contains one GST C-terminal domain. Both EF-1 beta and EF-1 delta stimulate the exchange of GDP bound to EF-1 alpha to GTP. Phosphorylation of EF-1 beta affects the GDP/GTP exchange rate. Alternative splicing events of the gene that encodes EF-1 beta result in three transcript variants that differ only in the 5' UTR. Applications: Suitable for use in Western Blot and ELISA. Other applications not tested. Recommended Dilution: Western Blot: 1:500-1:2000 ELISA: 1:20,000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.