D-3-phosphoglycerate dehydrogenase (FITC)

Size

£460.00

SKU:: 312981
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant human D-3-phosphoglycerate dehydrogenase protein
Format:: Supplied as a liquid in 0.01M PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol. Labeled with Fluorescein isothiocyanate (FITC).
Extra Details:: PHGDH or 3-phosphoglycerate dehydrogenase is an enzyme belonging to the D-isomer specific 2-hydroxyacid dehydrogenase family. It catalysis the reaction 3-phosphoglycerate + NAD+ = 3-phosphonooxypyruvate + NADH during L-serine biosynthesis. Defects in PHGDH are the cause of phosphoglycerate dehydrogenase deficiency (PHGDH deficiency). It is characterized by congenital microcephaly, psychomotor retardation and seizures. Applications: Suitable for use in FLISA and Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.