Cofilin1/Cofilin2 (CFL1/CFL2)

Size

£544.00

SKU:: 311460
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Peptide corresponding to the sequence around aa86-90 (A-T-Y-E-T) from human Cofilin1/Cofilin2.
Format:: Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 0.02% sodium azide, 50% glycerol.
Extra Details:: Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods. Applications: Suitable for use in Immunofluorescence, ELISA, Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilutions: Immunofluorescence: 1:100-1:200 Western Blot: 1:500-1:1000 Immunohistochemistry (paraffin): 1:50-1:200 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.