BTK (Phospho-Tyr223)

Size

£518.00

SKU:: 308310
Application:: WB
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Peptide corresponding to the sequence around phosphorylation site of tyrosine 223 (A-L-Y(p)-D-Y) derived from Human BTK.
Format:: Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 0.02% sodium azide, 50% glycerol.
Extra Details:: Non-receptor tyrosine kinase indispensable for B lymphocyte development, differentiation and signaling. Binding of antigen to the B-cell antigen receptor (BCR) triggers signaling that ultimately leads to B-cell activation. After BCR engagement and activation at the plasma membrane, phosphorylates PLCG2 at several sites, igniting the downstream signaling pathway through calcium mobilization, followed by activation of the protein kinase C (PKC) family members. PLCG2 phosphorylation is performed in close cooperation with the adapter protein B-cell linker protein BLNK. Sulekha Verma, J. Biol. Chem., Feb 2001; 276: 4671 - 4676. Yan M. Li, Cancer Res., Nov 2003; 63: 7630 - 7633. Malti Nikrad, Mol. Cancer Ther., Mar 2005; 4: 443 - 449. Feng Dong, Infect. Immun., Mar 2005; 73: 1861 - 1864. Hiroo Ueno, Mol. Biol. Cell, Feb 2 Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:500-1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice