Bcl-2 (BCL2, Apoptosis Regulator Bcl-2)

Size

£364.00

SKU:: 307714
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to human Bcl-2 around the non-phosphorylation site of T56.
Format:: Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Extra Details:: Bak is a 23kD homolog of the apoptosis inhibitor, Bcl-2 with an apparent molecular weight of 30kD on SDS-PAGE. Bak is a member of the Bcl-2 family of proteins and contains the bcl-2 homology regions (BH-) 1, 2 and 3. Unlike Bcl-2, Bak promotes apoptosis by interacting with and counteracting the anti-cell death effect of Bcl-2. Enforced expression of Bak induces rapid and extensive apoptosis of serum-deprived fibroblasts. However, Bak can inhibit cell death in an Epstein-Barr virus transformed cell line. Applications: Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunofluorescence: 1:200-1:1000 ELISA: 1:10,000 Western Blot: 1:500-1:2000 Immunohistochemistry: 1:100-1:300 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.